The process of heat sterilization of tools podiatric, dental or tattoo artists, ends with sterilization in electrical equipment such as sterilizers Dry heat and / or pressure tanks. These, to ensure the effectiveness of the sterilization carried out, they need periodic inspections and tests at each cycle. The autoclaves with saturated steam also, according to the UNI EN 29000, are subject to the annual periodic inspections by specialized companies that, by means of the sensors, verify the correct balance between pressure and temperature. The control of the sterilization process test to be performed in the other hand are divided into function test and control tests, or of verification of sterility, and each of them has its own timing and mode of application.
Test to be performed in the sterilization process
For the dry heat sterilizer (Pasteur or oven), in addition to the visual and practical controls that we can do on a daily basis such as cleaning, check the correct operation of the closing of the door and check the correct operating temperature, there are no official function test . The beauty center, or at least the professional who uses it in that moment, however, can still run tests that prove whether or not the successful infertility (control tests) that we discuss below.
The autoclave with saturated steam instead, mainly used by dentists and podiatrists, is subject to systematic periodic checks. There are in fact of daily control tests to be performed that are very important to document the correct functioning of the autoclave and of the individual under examination cycle. In addition, by law, it is obliged to have a registry where document every test carried out with general maintenance.
The control tests are divided into:
FUNCTION TEST - to ascertain the suitability of the autoclave;
TEST CONTROL of the sterilization - to avoid malfunctions and human errors in the execution of the cycle;
PERIODIC VALIDATION - to verify the performance of the autoclave
The function test
The function test allow to verify if the autoclave is able to ensure the essential operating performance, that is, the correct evacuation of the air and the consequent and total penetration of steam into the sterilization chamber. These tests are then allocated to the autoclave occurs with cycles in class D or B and can not be run on autoclaves with only cycles in class N, because the thermodynamic system does not allow for adequate air elimination.
The function tests are performed before using the autoclave, to verify their efficiency.
They are divided into:
VACUUM TEST (vacuum leak test) - Predicted in all autoclaves with loops in class B and some with class S cycles; usually the machine has a special program to check the degree of vacuum reached and its duration, and that these are sufficient.
TEST OF PENETRATION OF STEAM - Intended for all autoclaves cycles in class B and some with S. Class cycles for this test are used for external devices.
The test of the vacuum test is already inserted in autoclaves and has the objective to verify the perfect sealing of the sterilization chamber, that is, that do not occur decreases the degree of vacuum in the hydraulic circuit. The test, programmable in autoclaves with loops in class B, and in some in the S-class, plan to run the vacuum phase, then the room is kept isolated for a predetermined time, after which it is indicated whether the increase in pressure due the possible re-entry of air is less than a predetermined value. This is a daily test which is performed to start the sterilizer, before starting the sterilization cycles. If the printer is not present in the autoclave it is necessary to transcribe on a special register the data which are read on the display related to the pressure, temperature and running time.
The steam penetration tests are instead charged through external kits such as the Test Bowie + Dick and Helix-Test: Test Bowie & Dick
The Bowie & Dick Test allows to verify the correct evacuation of the air from the sterilization chamber and consequently, the complete penetration of steam into porous
loads (example: towels or gauze). The test consists of an indicator sheet, formed by concentric circles of light color, entered into a "test package" ready to use. After exposure the paper indicator changes to black.
The Helix-test will detect the strength of penetration of steam inside the chamber and the total expulsion of the air inside. Helix-heads a daily test which is
performed after the vacuum test, and earlier than normal autoclave use, this test should be done especially in the dental practice as there is the presence of instruments with cavities "A," that is, having a certain length and diameter of the cavity (vacuous voids with very fine lumi and of considerable depth such handpieces). The Helix-test must be performed each time the autoclave is started.
The control of the sterilization test
The control and test of the sterilization, allow to verify that the cycle at which the load, has been performed in the correct temperature / pressure parameters, saturated steam and time has been subjected.
The control and test of the sterilization, conjure operator error, as the excessive load of the sterilizing chamber, over the maximum weight indicated and the misspelled ta distribution of the same.
For the execution of tests are used appropriate sterilization indicators that will allow you to monitor all the autoclaves, with cycles in Class B, S or N, and also the dry heat sterilizers.
The indicators are divided into:
Chemical - are data from substances that color change color in relation to the temperature and / or the time for which has been maintained.
organic - using spores of germs apathogenic
The supplement migrant bar - Vapor Line is a chemical indicator which controls all of the sterilization parameters (temperature / pressure - time and presence of saturated steam Vapor Line) for saturated steam autoclaves. When inside the chamber it is reached temperature / pressure parameters and saturated steam, suitable for sterilization, to test internal wax starts to melt and advances coloring the bar (like a thermometer).
Strips or adhesive tapes indicators applicable to products to be sterilized. Allow to check the success of the sterilization. of black color bands will appear at the end of the sterilization. 3 models are available: the sterility indicator ribbon sterility 'autoclave indicator (humid heat) sterilization indicator strips for autoclave (moist heat) indicator tape for dry sterilization (for dry heat sterilizers)
The spore test is an indicator organic of the sterilization cycle. For saturated steam autoclaves are used spores of Bacillus Stearothermophilus because nonpathogenic and extremely resistant to heat. biological test of sterilitàSono vials containing a disk or special paper strip, where they were inoculated spores and an ampule containing the grounds of violet color culture. To the culture medium has been added a pH indicator. If at the end of the incubation period is not the case the growth of the spores, the pH and then the color will not change, demonstrating the successful sterilization. In the case of possible growth of the spores, the pH will decrease, causing the color change from violet to yellow highlighting the sterilization failure.
To every load that you are running you may want to place a chemical indicator of sterility inside a box, in the same conditions of the material that you sterilize. With this test the parameters are measured: temperature, pressure and exposure times. In addition, the same envelopes must have a chemical process indicator in order to check whether the packaging has been exposed to a sterilization cycle or biological meno.L'indicatore is a test that you should make at least once a year.